Cloned (Comment) | Organism |
---|---|
cloning of the cDNA by PCR. DNA encoding TaDH is amplified by PCR from Suberites domuncula cDNA library, using the primers that are designed to amplify the complete open reading frame. Expression in Escherichia coli as His-tag fusion protein. Two allelic variants are identified, which are present in the different specimens either in a homozygotic or in a heterozygotic way (length polymorphism (453 bp and 468 bp long) is detected in the second intron and the beginning of third exon). | Suberites domuncula |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | level of expression of TaDH is controlled by oxygen: TaDH is expressed when the oxygen level is low, during anaerobic glycolysis | Suberites domuncula |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36920 | - |
calculated from amino acid sequence | Suberites domuncula |
36920 | - |
deduced from cDNA and determined by SDS polyacrylamide gele electrophoresis and Western blot analysis | Suberites domuncula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Suberites domuncula | involvement of the sponge TaDH in the final step of the glycolytic pathway in the regeneration of NAD+ under anaerobic conditions | ? | - |
? | |
additional information | Suberites domuncula | opine production, opines are condensation products of an amino acid and a ketonic acid or a sugar | ? | - |
? | |
taurine + NADH + H+ + pyruvate | Suberites domuncula | taurine is involved in osmoregulation in marine animals | tauropine + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Suberites domuncula | B1GT63 | - |
- |
Suberites domuncula | B1GT63 | demosponge | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA-column chromatography | Suberites domuncula |
purification by metal-chelate affinity chromatography on a Ni-NTA-column | Suberites domuncula |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | sponges are collected in Croatia and whole bodys are used for enzyme characterization, high levels of this enzyme in most of cell types | Suberites domuncula | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | involvement of the sponge TaDH in the final step of the glycolytic pathway in the regeneration of NAD+ under anaerobic conditions | Suberites domuncula | ? | - |
? | |
additional information | opine production, opines are condensation products of an amino acid and a ketonic acid or a sugar | Suberites domuncula | ? | - |
? | |
taurine + NADH + H+ + pyruvate | taurine is involved in osmoregulation in marine animals | Suberites domuncula | tauropine + NAD+ + H2O | - |
? | |
Taurine + pyruvate + NADH | - |
Suberites domuncula | Tauropine + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SD_TaDH | - |
Suberites domuncula |
TaDH | - |
Suberites domuncula |
tauropine dehydrogenase | belongs to the family of opine dehydrogenases, phylogenetic analyses indicate that TaDH is only distantly related to the opine dehydrogenases from marine invertebrates, high sequence similarity to bacterial ornithine cyclodeaminases | Suberites domuncula |
tauropine [(carboxyethyl)-taurine/derived from sulfhydryl-amino acids] dehydrogenase | - |
Suberites domuncula |
tauropine [(carboxyethyl)-taurine/derived from sulfhydrylamino acids] dehydrogenase | - |
Suberites domuncula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | TaDH displays the GxGxxG/A motif known to be conserved among NAD(P)-dependent dehydrogenases | Suberites domuncula | |
NADH | - |
Suberites domuncula |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Suberites domuncula | theoretical value | - |
5.4 |
Suberites domuncula | calculated from amino acid sequence | - |
5.44 |